Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K3257109

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-15)


gag-Q-pol with ptetR Promoter

This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and ptetR. Under different regulation by different promoters, the expression level varies accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 259
    Illegal BglII site found at 1371
    Illegal BglII site found at 2677
    Illegal BglII site found at 3909
    Illegal BamHI site found at 2694
    Illegal BamHI site found at 3000
    Illegal XhoI site found at 1025
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1910
    Illegal AgeI site found at 3481
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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