Composite

Part:BBa_K3257108

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-15)


gag-Q-pol with J23119 Promoter-IPTG Inducible

This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and J23119 Promoter. Under different regulation by different promoters, the expression level varies accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal BglII site found at 1352
    Illegal BglII site found at 2658
    Illegal BglII site found at 3890
    Illegal BamHI site found at 2675
    Illegal BamHI site found at 2981
    Illegal XhoI site found at 1006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1891
    Illegal AgeI site found at 3462
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None