Composite

Part:BBa_K3257107

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-15)


gag-Q-pol with T7 Promoter-IPTG Inducible

This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and T5 Promoter. Under different regulation by different promoters, the expression level varies accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1336
    Illegal BglII site found at 2642
    Illegal BglII site found at 3874
    Illegal BamHI site found at 2659
    Illegal BamHI site found at 2965
    Illegal XhoI site found at 990
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1875
    Illegal AgeI site found at 3446
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None