Regulatory

Part:BBa_K3257076

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-13)

tRNA Primer

This part is key to the initiation of the reverse transcription process. MMLV reverse transcriptase starts to synthesis a new strand of cDNA with the help of tRNA for Proline in cells. We use the tRNAPro in E.coli to initiate reverse transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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