DNA

Part:BBa_K3257074

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-06)

lox2272

This part is a binding site for Cre recombinase and it is orthogonal to the native loxP site which means that it can only interact with the same lox2272 site rather than loxP.

Fig. 1 The verification of incompatibility between LoxP and different Lox sites. Plasmids containing the Cre gene were co-transformed respectively with plasmids containing mCherry flanked by a LoxP site and either LoxP, Lox2272 or Lox5171 site. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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