DNA

Part:BBa_K3257060

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)

PolyPurine Tract

Poly-purine tract is the part to pause the digestion of mRNA by RNaseH after the first strand transfer protecting the template strand from degradation and preparing for the second strand transfer. It is a region that won't be digested by the RNaseH domain, and serves as the primer for plus strand DNA synthesis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None