Coding

Part:BBa_K3255000

Designed by: Igor Martinez, Juan Nogales   Group: iGEM19_MADRID_UCM_HS   (2019-10-16)


Enzyme of the athocyanine route synthesis

Schematic illustration of the vector SEVA 181 containing F3H (flavanone3-hydroxylase) gene flanked by Bsa I recognition sites.

Our part codifies for enzyme flavanone3-hydroxylase in the route of anthocyanin synthesis that transforms naringenin in dihydrokaempferol. It has a plant origin, from Camelia sinensis. Codons had been optimized for E. coli. It contains fusion sites for Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None