Translational_Unit

Part:BBa_K325211

Designed by: Bill Collins and Peter Emmrich   Group: iGEM10_Cambridge   (2010-10-23)

Orange Firefly Luciferase
L. Cruciata
(E. coli optimised)

Input: L-Arabinose
Output: Light (orange)

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     Add Data       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Ser286Asn mutation was used to create a orange bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325249 against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1160


[edit]
Categories
Parameters
None