Composite

Part:BBa_K3252044

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Full system integration of LuxOUNgRNA-dCas9GFPmut3b

BBa_K3252044 is full system integration of LuxOUNgRNA-dCas9GFPmut3b. This system was evaluate with react this system with AI that produced by Vibrio parahaemolyticus. Consentration of AI was assumed by the supernatant delution over nigh cuture of V. parahaemolyticus with our system integration. The GFPmut3b that produced was observed during a certain time.

Based our work the best result is 50% (v/v)delution of V. parahaemolyticus supernatant with our system integration growth media and one hour incubation period. See our result page to for detailed information.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7598
    Illegal NheI site found at 6080
    Illegal NheI site found at 7357
    Illegal NheI site found at 10524
    Illegal NheI site found at 10547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7598
    Illegal BglII site found at 4254
    Illegal BamHI site found at 9636
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 11222
    Illegal SapI site found at 853
    Illegal SapI site found at 2457


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Categories
Parameters
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