Inverter

Part:BBa_K3252043

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


dCas9-GFPmut3b Inverter Module

Part BBa_K3252043 is actuator module that consist of BBa_K3252040 (dCas9) and BBa_K3252041 (GFPmut3b). This inverter module will express dCas protein that will build dCas9-gRNA complex which is will repress J23115 promoter (BBa_K3252023) that regulate the expression of GFPmut3b (BBa_K3252041).

Characterization of this part was evaluate in full system integration part (BBa_K3252044).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1447
    Illegal NheI site found at 1206
    Illegal NheI site found at 4373
    Illegal NheI site found at 4396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1447
    Illegal BamHI site found at 3485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5071


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