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Part:BBa_K3252042

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


LuxOUN-gRNA Actuator Module

Part BBa_K3252042 is actuator module that consist of BBa_K3252036 (LuxO), BBa_K3252037 (LuxU), BBa_K3252038 (LuxN), and BBa_K3252047 (gRNA). This actuator module will express Lux cascade protein that will produce gRNA in low consetration of AI.

Characterization of this part was evaluate in full system integration part (BBa_K3252044).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 853
    Illegal SapI site found at 2457


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Parameters
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