Composite

Part:BBa_K3252037

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Pr-RBS-LuxU-Term

Part BBa_K3252037 is basic composite part that express LuxU protein. LuxU protein is one of the phosphorelated protein that used in Qrr RNA Lux cascade system in Vibrio parahaemolyticus. LuxU will phosporelated when integral protein (LuxN, Cqss, LuxP) active (phosporelated). BBa_K3252037 was controled under p66 constitutive promoter (BBa_K3252021) and weak RBS BBa_K3252026. BBa_K3252037 was assemble with thats part and BBa_K3252034 transcription terminator by ordering custom gene synthetic.

Characterization of this part was perform by full system integration BBa_K3252044 transcription unit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 854


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Categories
Parameters
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