Coding

Part:BBa_K3252032

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


dCas9 coding squence

Part BBa_K3252032 is dCas9 coding sequence that used to express dCas9 protein. dCas9 protein with some unique gRNA will build dCas9-gRNA complex that can repress promoter gene region. This coding sequence has been assemble in pdCas9 plasmid backbond.

dCas9 (BBa_K3252032) was constructed in BBa_K3252040 transcription unit with BBa_K3252025 RBS (BBa_B0034), BBa_K3252022 (GabDP2 constitutive promoter) and BBa_K3252034 transcription terminator (BBa_B0015).

Characterization of this parts was perform in our system integration transcription unit BBa_K325244.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None