Reporter

Part:BBa_K3252031

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


GFPmut3b coding sequence BBa_E0040

Part BBa_K3252031 is the GFPmut3b coding sequence (BBa_E0040) that we used for reporter gene. In our iGem team project this part has been assemble in BBa_K3252041 transcription unit with BBa_K3252025 RBS (BBa_B0034), BBa_K3252023 (BBa_J23115 constitutive promoter), and BBa_K3252034 transcription terminator (BBa_B0015). That parts were assembled by order custom gene synthetic.

Characterization of this part was performed with the transcriptional unit BBa_K3252041. By using our experimental protocol, we characterize this expression level which is growth in different level of salinity. We cultivate Escherichia coli TOP10 that carrying BBa_K3252041 in LB media with different level of salinity.

1. LB media with 10 ppt

2. LB media with 25 ppt

3. LB media with 35 ppt

4. LB media with 45 ppt

5. LB media with sea water

Different level of salinity will influence the cell growth and the level of expression of some gene. In this case expresssion level of GFPmut3b was influence by the cell growth or OD600 that we observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None