Coding

Part:BBa_K3252029

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


V. parahaemolyticus LuxU protein coding sequence

Parts BBa_K3252029 is coded LuxU protein. LuxU protein is a phosporelated cascade protein that expressed in Vibrio parahaemolyticus. This protein play role as phosphorelated protein receptor from phosphorelated LuxN protein when the cell density of V. parahaemolyticus is low. This protein act as posporelated cascade to regulate the pQrr promoter that will be express the transcription factor to drive the expression from biomass cultivation to produce toxin in Vibrio parahaemolyticus when the cell density is high.

LuxU has been assembled with BBa_K3252021 as promoter, BBa_K3252026 as RBS, and BBa_K3252034 as terminator to build BBa_K3252037 as transcription unit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None