Coding

Part:BBa_K3252028

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


V. parahaemolyticus LuxO protein coding sequence

Parts BBa_K3252028 is coded LuxO protein. LuxO protein is a phosporelated cascade protein that expressed in Vibrio parahaemolyticus. This protein play role as phosphorelated protein receptor from phosphorelated LuxU protein. This protein act as posporelated cascade to activate the pQrr promoter that will be express the transcription factor to drive the expression from biomass cultivation to produce toxin in Vibrio parahaemolyticus when the cell density is high.

LuxO has been assembled with BBa_K3252021 as promoter, BBa_K3252025 as RBS, and BBa_K3252034 as terminator to build BBa_K3252036 as transcription unit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 770


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Parameters
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