Regulatory

Part:BBa_K3252023

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Constitutive promoter BBa_J23115

Part BBa_K3252023 is the constitutive promoter BBa_J23115 that we used for regulate the GFPmut3b (BBa_K3252011) expression as reporter gene. BBa_J23115 is one of the medium to strong Andersons promoter collections. In our iGem team project this promoter has been assemble in BBa_K3252041 transcription unit with BBa_K3252025 RBS (BBa_B0034) and BBa_K3252034 transcription terminator (BBa_B0015). Thats parts were assembled by order custom gene synthetic.

Characterization of this part was performed with the transcriptional unit BBa_K3252041. By using our experimental protocol, we characterize expression level which is regulated by this part in different level of salinity. We cultivate Escherichia coli TOP10 that carrying BBa_K3252041 in LB media with different level of salinity.

1. LB media with 10 ppt

2. LB media with 25 ppt

3. LB media with 35 ppt

4. LB media with 45 ppt

5. LB media with sea water

Different level of salinity will influence the cell growth and the level of expression of some gene. In this case expresssion level of GFPmut3b was influence by the cell growth or OD600 that we observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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