Regulatory

Part:BBa_K3252022

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Constitutive promoter GabDP2

Part BBa_K3252022 is the constitutive promoter GabDP2 that used to regulate the dCas9 protein expression. This promoter has been assemble in pdCas9 plasmid backbond.

This promoter was used to regulate dCas9 protein (BBa_K3252032) in BBa_K3252040 transcription unit with BBa_K3252025 RBS (BBa_B0034) and BBa_K3252034 transcription terminator (BBa_B0015). That parts were assembled by ordering custom gene synthetic.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None