Regulatory

Part:BBa_K3252021

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Constitutive promoter p66

Part BBa_K3252021 is the constitutive promoter p66 that used to regulate the Lux cascade protein expression. This promoter was used to express LuxO, LuxU, and LuxN protein (BBa_K3252028-BBa_K3252030) in BBa_K3252036-BBa_K3252038 transcription unit with BBa_K3252025-BBa_K3252027 RBS (BBa_B0034, BBa_B0032, BBa_B0033) and BBa_K3252015 transcription terminator (BBa_B0015). They all were assembled by ordering custom gene synthetic.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None