Composite

Part:BBa_K324052

Designed by: Ethan Richman   Group: iGEM10_Brown   (2010-10-25)


pLac/Mnt - RBS - GAL4 - ECFP - Term

This part is composed of the pLac/Mnt hybrid promotor with GAL4 and ECFP following. Expression occurs in the absence of Mnt + presence of LacI and IPTG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal XhoI site found at 1
    Illegal XhoI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 249


[edit]
Categories
Parameters
None