Composite
Part:BBa_K324051
Designed by: Ethan Richman Group: iGEM10_Brown (2010-10-25)
LacI RFP Bistable part GFP
This is the Bistable part originally created by team PKU '07, modified by PKU '09, and now further modified by us. We have added expression of LacI before RFP and the CI434 regulated promotor. As described in No part name specified with partinfo tag., and detailed [http://2007.igem.org/Peking_Push-on-push-off#Constructing_the_Bistable_Switch here] this part has two stable states. Initially, the green, or CI434 state dominates. However, as CI is introduced, expression of CI434 (and thus state 1) is inhibited, and the bistable part switches to state 2, where CI and RFP are produced. We have added the additional expression of LacI in state 2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1147
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1171
Illegal AgeI site found at 1283 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |