Composite

Part:BBa_K324051

Designed by: Ethan Richman   Group: iGEM10_Brown   (2010-10-25)


LacI RFP Bistable part GFP

This is the Bistable part originally created by team PKU '07, modified by PKU '09, and now further modified by us. We have added expression of LacI before RFP and the CI434 regulated promotor. As described in No part name specified with partinfo tag., and detailed [http://2007.igem.org/Peking_Push-on-push-off#Constructing_the_Bistable_Switch here] this part has two stable states. Initially, the green, or CI434 state dominates. However, as CI is introduced, expression of CI434 (and thus state 1) is inhibited, and the bistable part switches to state 2, where CI and RFP are produced. We have added the additional expression of LacI in state 2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1171
    Illegal AgeI site found at 1283
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None