Composite

Part:BBa_K324004

Designed by: Julius Ho   Group: iGEM10_Brown   (2010-10-25)


AraC inducible ChFP generator

This part combines two previously available BioBricks via standard assembly: a promoter inducible by AraC (BBa_R0080) and CherryFP generator (BBa_J06702). This composite acts a reporter in E. coli, allowing cells to produce cherry fluorescent protein in the presence of AraC protein. In the absence of AraC, minimal levels of fluorescence should be observed.

The cherry FP generator consists of an RBS, mCherry, and double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None