Part:BBa_K324002

Tat protein transduction domain with glycine linker (RFC25)

This part consists of the Tat protein transduction domain, an 11 a.a. sequence able to carry attached proteins across cell membranes without need to permeabilize. This domain, attached to a glycine linker, can be joined to other peptides in the RFC25 Freiburg assembly format to create fusion proteins that can freely transduce cell membranes. Our part consists of the Tat-glycine linker, intended to be attached to the N-terminus of any fusion protein construct.

The original Tat a.a. sequence comes from the Tat protein in human immunodeficiency virus but does not possess any innately pathogenic features. Bsl2 safety precautions are generally recommended for Tat-fusion proteins, although Tat DNA can be manipulated without special protocols.

We originally obtained the Tat-PTD DNA from Will Donovan (william_donovan@brown.edu)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]