Part:BBa_K3239003

Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain

Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain. Derepressed by Mxr1 and upregulated by Mit1 and Prm1.

Usage and Biology

The AOX1 promoter (P_AOX1) is homogeneous to the P. pastoris GS115 strain. It is derepressed by Mxr1 and upregulated by Mit1 and Prm1, all transcription factors homogeneous to the P. pastoris GS115 strain.

This part should be identical to the alcohol oxidase promoter registered as BBa_K431007, except that this our part (BBa_3239003) is also RFC 21 compatible.

Functional Parameters

Published literature has shown that this promoter is tightly regulated by its transcription factors and has a very low leakiness.

Reference

Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738

Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014

Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]