Composite

Part:BBa_K3237024

Designed by: Luke Saville, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-09)


NAB1

NAB1 is a RNA binding protein in photorophic organisms that specifically suppress the translation of light harvesting antenna proteins [1,2]. These light antenna change in size in order to efficiently capture more light for energy. This part is designed to be expressed in Chlamydomonas reinhardtti to cause a decrease in antenna size. Studies have shown that more light is needed for growth[1,2]. This construct is a control construct for our antisense NAB1 consruct BBa_K3237012 which is proposed to be used to allow for algal growth under lower light conditions.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1535
    Illegal NheI site found at 1087
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
    Illegal NotI site found at 1571
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1535
    Illegal BamHI site found at 1553
    Illegal XhoI site found at 577
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None