Composite

Part:BBa_K3237015

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-05)


His-mRFP1 codon optimized for C. reinhardtii

This part is based on the mRFP1 part BBa_E1010. A hexa-histidine tag was added onto the N-terminus for purification purposes. As the project is based on the standardization of insulin production using RFP, we added the his tag in order to use the RFP protein as a control for fluorescent measurement as well as insulin purification. The expression of insulin was also designed to be implemented into microalgae and is therefore codon optimized for Chlamydomonas reinhardtii .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1502
    Illegal NheI site found at 1054
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
    Illegal NotI site found at 1538
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1502
    Illegal BamHI site found at 1520
    Illegal XhoI site found at 656
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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