Coding

Part:BBa_K3237000

Designed by: Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-03)


Insulin Receptor Protein

The insulin receptor protein is a regulator of glucose transport in and out of cells by its interaction with the presence of insulin protein[1]. This construct was designed with a N-terminal His tag for affinity purification and was placed under constitutive expression as seen with part BBa_K3237013. This part was created in order to perform binding assays and to confirm proper processing/activity of insulin constructs made by the 2019 University of Lethbridge iGEM team. The part was also optimized for expression in E.coli to obtain the quickest yields as a opposed to microalgae expression which our project proposes for insulin production. Our data for this part can be found on the composite parts page for BBa_K3237013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None