Device

Part:BBa_K323115

Designed by: Nejc Tomšič   Group: iGEM10_Slovenia   (2010-10-20)

pT7_TetO+NLS_T7RNAP_CL1_PEST

T7 RNA polymerase with NLS, CL1 and PEST tags under T7 promoter with Tet operator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2729
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2729
    Illegal NgoMIV site found at 2823
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None