Part:BBa_K323005
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system
iGEM 2010 team Slovenia used this part along with 4 others [BBa_K323021, BBa_K323029, BBa_K323080, BBa_323039] to exhibit FRET reconstitution on a predefined DNA scaffold in vivo by transfecting mammalian HEK293 cell line with these 5 plasmids. In particular this BioBrick is part of a FRET subsystem leading to split mCitrine reassembly. It consists of PBSII synthetic zinc finger DNA binding domain, a linker sequence and N terminal fragment of mCitrine. Reconstitution of a fluorescent protein was observed under confocal microscope. mCitrine was excited with 514nm Argon laser and emmision was detected in 520 to 580 nm window. Combining this 2-part subsystem with the other one, leading to mCerulean reassembly [BBa_K323021, BBa_K323029] FRET on a DNA program can be observed.
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Check out more at [http://2010.igem.org/Team:Slovenia Team Slovenia]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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