Sequence and Features
- 10INCOMPATIBLE WITH RFCIllegal XbaI site found at 1186
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23INCOMPATIBLE WITH RFCIllegal XbaI site found at 1186
- 25INCOMPATIBLE WITH RFCIllegal XbaI site found at 1186
- 1000INCOMPATIBLE WITH RFCIllegal SapI.rc site found at 242
This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of iGEM Marburg 2019.
These parts are compatible with the RCF  standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.