Coding

Part:BBa_K3228100

Designed by: Marian Krämer   Group: iGEM19_Marburg   (2019-10-18)


pMC_0_4_35_cpf1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1713
    Illegal NheI site found at 3433
    Illegal SpeI site found at 3622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from Francisella tularensis ssp. novicida, which has been adapted to the MoClo standard (Ungerer & Pakrasi, 2016).

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.

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Categories
Parameters
None