pMC_1_002_characterization_RBS_S.e.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3526
Illegal PstI site found at 6061 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 427
Illegal NheI site found at 450
Illegal PstI site found at 6061
Illegal NotI site found at 2328
Illegal NotI site found at 8065 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 7224
Illegal XhoI site found at 3576 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3526
Illegal PstI site found at 6061 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3526
Illegal PstI site found at 6061
Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2375
Illegal NgoMIV site found at 2517 - 1000COMPATIBLE WITH RFC[1000]
Description
This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range BBa_K3228073 to BBa_K3228075 as well as BBa_K3228090) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria BBa_K3228069 and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder (compare BBa_K3228060) for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.
In our resultswe show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility. For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for Vibrio natriegens in the last year.
Compability
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
