Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
This part contains the ribosomal binding site B0034 and PlmJ which is a ribozyme. PlmJ comprises the ribozyme with an additional hairpin to help expose the RBS. PlmJ is an insulator equally effective as RiboJ (Lou, Stanton, Chen, Munsky, & Voigt, 2012).
Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).
This leads to a more stable expression independent of the cellular context (See iGEM William and Mary 2016) and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
These parts are compatible with the RCF  standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.