pMC_0_4_25_Antares2 (S.e.)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1513
Illegal PstI site found at 1409 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1513
Illegal PstI site found at 1409 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1513
Illegal PstI site found at 1409 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1513
Illegal PstI site found at 1409 - 1000COMPATIBLE WITH RFC[1000]
Description
Antares2 is a coupled bioluminescence protein consisting of TeLuc and two flanking CyOFP fluorescence reporters that abuses the Bioluminescence Resonance Energy Transfer (BRET) to excite CyOFP with the luminescence of TeLuc.
This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
experimental dataand literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
Compability
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
