pMC_0_4_22_NanoLuc-K1159001
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 520 - 1000COMPATIBLE WITH RFC[1000]
Description
NanoLuc is a small luminescent reporter with just a molecular weight of 19,5 kDA. This reporter stands out with a signal strength that is orders of magnitude higher than compared traditional luminescent reporters.
This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
experimental dataand literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
Compability
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
