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Part:BBa_K322710

Designed by: Chris French and Maria Kowal   Group: iGEM10_Edinburgh   (2010-10-27)


Marker construct which can be used to target tnaA in E.coli.

This is a composite part containing the positive selection marker cat (chloramphenicol resistance) and the negative selection marker sacB (lethal to host in the presence of sucrose) fused to the up and down stream sequences of tnaA, the indole-producing gene. This construct can be used in the BRIDGE protocol for targeted deletion/replacement of tnaA in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None