Coding

Part:BBa_K322246

Designed by: Meng Lu and William Rostain   Group: iGEM10_Edinburgh   (2010-09-24)

Red emitting firefly luciferase mutant (S284T)

Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter than BBa_K322211.

In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


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Categories
Parameters
None