Composite

Part:BBa_K322150

Designed by: Chris French and Richard Partridge Hicks   Group: iGEM10_Edinburgh   (2010-10-08)


Bacterial luciferase (luxAB) blue shifted by lumazine protein, with promoter

Bacterial luciferase from X. luminescens (luxAB) with Lumazine protein which shifts its spectrum towards blue. Note that LuxAB uses decanal as a substrate, this needs to be added or synthesised by the bacteria (Decanal production is coded for by BBa_K322312).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3080
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1657


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Categories
Parameters
None