Project

Part:BBa_K3218008

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-16)


CRISPR-X: deactivated Cas9 coupled to activation induced deaminase (AID)

A gene encoding a mutant MS2 coat protein (BBa_K3218006) with a nuclear localization signal fused to an Activation-induced Deaminase (AID). The gene also encodes a dCas9 protein used to target specific loci within DNA. MS2 protein is used to attract the MS2-AID complex to the hairpin loops on the gRNA. In this manner the AID is brought to the target locus within the DNA. An improved version of MS2 protein is encoded which has a higher affinity for the MS2 hairpin loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1992
    Illegal BglII site found at 2451
    Illegal BglII site found at 2540
    Illegal BglII site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 498
    Illegal SapI site found at 599


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Categories
Parameters
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