Coding

Part:BBa_K3206012

Designed by: Karen Fung   Group: iGEM19_Newcastle   (2019-10-16)


His-tagged eGFP

The part BBa_K2680546 eGFP fluorescent protein has been His-tagged at the C-terminal to allow for protein purification using the His-trap. Purified protein can then be used to formulate a standard curve of the fluorescence level of the protein concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
    Illegal BamHI site found at 721
    Illegal BamHI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None