Composite
Part:BBa_K3206008
Designed by: Matthew Rogan Group: iGEM19_Newcastle (2019-10-15)
Constitutively expressed GcvA gene
This composite part consists of a BBa_J23100 promoter, which allows constitutive expression of the transcription factor GcvA (BBa_K3206004). GcvA encodes the glycine cleavage activator transcription factor, which is involved in the activating expression of genes in the glycine cleavage operon by binding to the promoter region of these genes. GcvA transcription factor is inactive under low levels of glycine due to GcvR binding to it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 599
Illegal BamHI site found at 907 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
transcriptional
transcriptional
biology | Escherichia coli K12 |
protein | GcvA |