Composite

Part:BBa_K3206008

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


Constitutively expressed GcvA gene

This composite part consists of a BBa_J23100 promoter, which allows constitutive expression of the transcription factor GcvA (BBa_K3206004). GcvA encodes the glycine cleavage activator transcription factor, which is involved in the activating expression of genes in the glycine cleavage operon by binding to the promoter region of these genes. GcvA transcription factor is inactive under low levels of glycine due to GcvR binding to it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 599
    Illegal BamHI site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biologyEscherichia coli K12
proteinGcvA