Composite

Part:BBa_K3206007

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


Constitutively expressed PepD gene

This transcriptional unit was made to constitutively express the gene PepD, which encodes a cytosol non-specific dipeptidase, using the constitutive promoter BBa_J23100. This protein hydrolyses the peptide bond formed between dipeptides e.g. cysteine-glycine dipeptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 254
    Illegal AgeI site found at 1407
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
biologyescherichia coli K12
proteinPepD