Regulatory

Part:BBa_K3202003

Designed by: Heyuan Ni   Group: iGEM19_BHSF_ND   (2019-10-14)


pBAD Promoter

A promotor

Sequence and Features<


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56

Contribution of SCAU-China 2023

To validate the effectiveness of our quorum-sensing-based T4 lysis device, we introduced the inducible araBAD promoter.. (Plasmid maps can be found in Figure 1)

part-1-1-6-1.png

Figure 1. Diagram of Quorum sensing-based T4 lysis device verification systems circuit design

We characterized the component to demonstrate its effectiveness, as detailed in the construction and characterization section in BBa_K4632024[1]

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