Generator
ori+Amp

Part:BBa_K320011

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)

Origin for Asaia and resistance to Ampicillin

This BioBrick contains an origin of replication for Asaia and E. coli (BBa_K320000) and a resistance to Ampicillin (BBa_P1002). It was initially designed to be inserted into (BBa_I51020). Warning: Don't try to use this in Asaia. Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


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Parameters
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