Composite

Part:BBa_K3196035

Designed by: XinRan Rao   Group: iGEM19_HUST-China   (2019-10-16)


Pgas-GFP

This is a composite part to check the function of the promoter gas, we can measure the fluorescence intensity at different pH to test it.Pgas, a low-pH-inducible promoter, promoted gene expression efficiently at low pHs, such as pH 2.0. After testing the promoter, replace the GFP protein with the target gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 51
    Illegal BamHI site found at 687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None