DNA

Part:BBa_K319035

Designed by: Afnan Azizi   Group: iGEM10_uOttawa   (2010-10-26)


URA3 ORF+URA3 terminator

This is the native S. cerevisiae URA3 cassette excluding the promoter. It can be used as an auxotrophic marker with a number of promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 669
    Illegal SapI.rc site found at 516


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Categories
Parameters
None