Part:BBa_K3187023
Tobacco Etch Virus (TEV) Protease Cleavage Site
Profile
| Name | Tobacco Etch Virus (TEV) Protease Cleavage Site |
| Base pairs | 21 |
| Molecular weight | 871 Da |
| Origin | Tobacco Etch Virus (TEV) |
| Properties | recognition site for the TEV protease |
Usage and Biology
The TEV protease cleavage site is the recognition sequence for a highly specific protease originated from the tobacco etch virus. The TEV protease recognizes the amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between Gln and (Gly/Ser) [1] [2] . We are using this recognition site to cut our protein in front of its polyG-tag so it has a C-terminal polyG-tag that can be used in a Sortase A reaction. [3] [4] [5] .
References
- ↑ J C Carrington and W G Dougherty, A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci U S A. 1988 May; 85(10): 3391–3395. [1]
- ↑ Product description TEV Protease, New England Biolabs [2]
- ↑ Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering [3]
- ↑ Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation [4]
- ↑ Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering [5]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |