Coding

Part:BBa_K3187022

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)


Superfolder Green Fluorescence Protein

Profile

Name sfGFP (superfolder green fluorescent protein)
Base pairs 711
Molecular weight 26.8 kDa
Origin Aequorea victoria
Properties very fast maturing variant of GFP

Usage and Biology

GFP is a fluorescent protein originally isolated from the jellyfish Aequorea victoria. sfGFP is a variant of this protein. It has a short maturing time of 13.6 min instead of 36.1 min of the original protein variant, an extinction maximum at 485 nm and an emission maximum at 510 nm [1] [2] [3] .

References

  1. ↑ Jean-Denis Pédelacq, Stéphanie Cabantous, Timothy Tran, Thomas C Terwilliger, Geoffrey S Waldo, Engineering and characterization of a superfolder green fluorescent protein, Nature Biotechnology volume24,pages79–88 (2006) [1]
  2. ↑ FPbase: Superfolder GFP, last visited: 6.10.2019 [2]
  3. ↑ Balleza, E., Kim, J. M., & Cluzel, P. (2018). Systematic characterization of maturation time of fluorescent proteins in living cells. Nature methods, 15(1), 47–51. [3]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


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Parameters
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