Composite

Part:BBa_K3187014:Design

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)


Plac-RBS-mCherry-double terminator (IPTG-inducible) with Translation Enhancing 5'UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Original version of the part was ordered from Integrated DNA Technologies (IDT) and cloned in pSB1C3 via Gibson Assembly. Improved version was created by primer hybridization (sequence of new 5'-UTR) and cloned via gibson assembly.

References