Terminator

Part:BBa_K3183023

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-10-16)

T1 terminator rrnB

Double terminator contains the T7 and terminator. This has been shown in the literature to be effective in L. reuteri constructs in conjunction with the T1 rrb terminator,1 but more work would be necessary to determine whether it is effective in Lactobacillus species alone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Lubkowicz, David, et al. “Reprogramming Probiotic Lactobacillus Reuteri as a Biosensor for Staphylococcus Aureus Derived AIP-I Detection.” ACS Synthetic Biology, vol. 7, no. 5, 2018, pp. 1229–1237., doi:10.1021/acssynbio.8b00063.

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Categories
Parameters
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